|
|
|
|
|
| Corbeil, Serge; Arzul, Isabelle; Diggles, Ben; Heasman, Mike; Chollet, Bruno; Berthe, Franck; Crane, Mark St. J.. |
| The development of molecular diagnostic assays with increased sensitivity compared with conventional histological techniques is highly desirable for effective management of bonamiosis in cultured oyster stocks and wild populations. A real-time TaqMan PCR assay was developed for the specific detection of Bonamia species in infected oyster tissues. The TaqMan assay was shown to be significantly more sensitive than histopathology. Although a real-time TaqMan PCR assay is comparable with conventional PCR in terms of sensitivity, it offers the advantages that it is a rapid test and has a very low risk of sample cross-contamination. Furthermore, it can be optimised to quantify the parasite load in samples. The assay detected Bonamia isolates from Australia, New... |
| Tipo: Text |
Palavras-chave: Oyster; Real time TaqMan PCR assay; Bonamia spp.. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1980.pdf |
| |
|
| |
|
|
| Gardner, Ian A.; Whittington, Richard J.; Caraguel, Charles G. B.; Hick, Paul; Moody, Nicholas J. G.; Corbeil, Serge; Garver, Kyle A.; Warg, Janet V.; Arzul, Isabelle; Purcell, Maureen K.; Crane, Mark St J.; Waltzek, Thomas B.; Olesen, Niels J.; Gallardo Lagno, Alicia. |
| Complete and transparent reporting of key elements of diagnostic accuracy studies for infectious diseases in cultured and wild aquatic animals benefits end-users of these tests, enabling the rational design of surveillance programs, the assessment of test results from clinical cases and comparisons of diagnostic test performance. Based on deficiencies in the Standards for Reporting of Diagnostic Accuracy (STARD) guidelines identified in a prior finfish study (Gardner et al. 2014), we adapted the Standards for Reporting of Animal Diagnostic Accuracy Studies—paratuberculosis (STRADAS-paraTB) checklist of 25 reporting items to increase their relevance to finfish, amphibians, molluscs, and crustaceans and provided examples and explanations for each item. The... |
| Tipo: Text |
Palavras-chave: Reporting standards; Sensitivity; Specificity; Finfish; Amphibians; Molluscs; Crustaceans; STRADAS-paraTB; Diagnostic validation. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00334/44505/44191.pdf |
| |
|
|
| Arzul, Isabelle; Corbeil, Serge; Morga, Benjamin; Renault, Tristan. |
| Although a wide range of viruses have been reported in marine molluscs, most of these reports rely on ultrastructural examination and few of these viruses have been fully characterized. The lack of marine mollusc cell lines restricts virus isolation capacities and subsequent characterization works. Our current knowledge is mostly restricted to viruses affecting farmed species such as oysters Crassostrea gigas, abalone Haliotis diversicolor supertexta or the scallop Chlamys farreri. Molecular approaches which are needed to identify virus affiliation have been carried out for a small number of viruses, most of them belonging to the Herpesviridae and birnaviridae families. These last years, the use of New Generation Sequencing approach has allowed increasing... |
| Tipo: Text |
Palavras-chave: Molluscs; Viruses; Diseases; Herpesviridae; Bivalve; Abalone. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00371/48206/48319.pdf |
| |
|
|
|
